Hypoxia activates constitutive luciferase reporter constructs
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چکیده
منابع مشابه
Assessment of immunity to mycobacterial infection with luciferase reporter constructs.
Protective immunity to mycobacterial infection is incompletely understood but probably involves the coordinated interaction of multiple cell types and cytokines. With the aim of developing assays that might provide a surrogate measure of protective immunity, we have investigated the use of recombinant mycobacteria carrying luciferase reporter enzymes to assess the effectiveness of antimycobacte...
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Luciferase is used as a reporter enzyme to estimate gene expression in prokaryotic or eukaryotic cells because the amount of luciferase present is correlated with light intensity in the presence of excess luciferin (1). Furthermore, its sensitivity and range of linear responses are superior to those of other typical reporters (2). When the luciferase gene containing a target promoter region is ...
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ژورنال
عنوان ژورنال: Biochimie
سال: 2011
ISSN: 0300-9084
DOI: 10.1016/j.biochi.2010.10.009